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Recombinant Human FGF-1/FGFa Protein
SKU: PKSH032430-50
Recombinant Human FGF-1/FGFa Protein
| SKU # | PKSH032430 |
| Expression Host | E.coli |
Description
| Synonyms | Acidic Fibroblast Growth Factor, ECGFHeparin-Binding Growth Factor 1, Endothelial Cell Growth Factor, FGF-1, FGF1, FGFA, Fibroblast Growth Factor 1, HBGF-1, aFGF |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala2-Asp155 |
| Accession | P05230 |
| Calculated Molecular Weight | 17.3 kDa |
| Observed Molecular Weight | 17 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
FGF acidic; also known as ECGF; FGF-1and HBGF-1; is a non-glycosylated heparin binding growth factor that is expressed in the brain; kidney; retina; smooth muscle cells; bone matrix; osteoblasts; astrocytes and endothelial cells. It is a mitogenic peptide that is produced by multiple cell types and stimulates the proliferation of cells of mesodermal; ectodermal; and endodermal origin. Its association with heparan sulfate is a prerequisite for activation of FGF receptors. Internalized FGF acidic migrates to the nucleus where it is phosphorylated by nuclear PKC delta; exported to the cytosol; dephosphorylated; and degraded. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling.