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Recombinant Human G-CSFR/CD114 Protein (Fc Tag)(Active)
SKU: PKSH031749-100
Recombinant Human G-CSFR/CD114 Protein (Fc Tag)(Active)
| SKU # | PKSH031749 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD114, CSF3R, G-CSF R, GCSFR |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Pro 621 |
| Accession | NP_000751.1 |
| Calculated Molecular Weight | 93.3 kDa |
| Observed Molecular Weight | 120-130 kDa |
| Tag | C-hFc |
| Bio-activity | Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this ettect is typically 2-20 ng/ml in the presence of 0.125ng/ml of recombinant human GCSF |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.