Recombinant Human G-CSFR/CD114 Protein (His Tag)(Active)
SKU: PKSH031748-100
Recombinant Human G-CSFR/CD114 Protein (His Tag)(Active)
SKU # | PKSH031748 |
Expression Host | HEK293 Cells |
Description
Synonyms | CD114, CSF3R, G-CSF R, GCSFR |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Met 1-Pro 621 |
Accession | NP_000751.1 |
Calculated Molecular Weight | 68.0 kDa |
Observed Molecular Weight | 92 kDa |
Tag | C-His |
Bio-activity | Measured by its ability to inhibit the GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this ettect is typically 50-250 ng/mL in the presence of 0.125ng/mL of recombinant human GCSF. |
Properties
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.