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Recombinant Human GALE Protein (His Tag)
SKU: PKSH033193-50
Recombinant Human GALE Protein (His Tag)
| SKU # | PKSH033193 |
| Expression Host | E.coli |
Description
| Synonyms | GALE, Galactowaldenase, UDP-Galactose 4-Epimerase, UDP-Glucose 4-Epimerase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ala348 |
| Accession | Q14376 |
| Calculated Molecular Weight | 40.4 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 2mM DTT, 1mM EDTA, pH 8.0. |
| Reconstitution | Not Applicable |
Background
The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.