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Recombinant Human Gastric Lipase/LIPF Protein (Baculovirus, His Tag)
SKU: PKSH030532-100
Recombinant Human Gastric Lipase/LIPF Protein (Baculovirus, His Tag)
| SKU # | PKSH030532 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | GL, Gastric Lipase, Gastric Triacylglycerol Lipase, HGL, HLAL, LIPF |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-Lys398 |
| Accession | AAI12273.1 |
| Calculated Molecular Weight | 44.6 kDa |
| Observed Molecular Weight | 45 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene.