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Recombinant Human Gastric Lipase/LIPF Protein (Human Cells, His Tag)
SKU: PKSH032480-50
Recombinant Human Gastric Lipase/LIPF Protein (Human Cells, His Tag)
| SKU # | PKSH032480 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | GL, Gastric Lipase, Gastric Triacylglycerol Lipase, HGL, HLAL, LIPF |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu20-Lys398 |
| Accession | AAI12273.1 |
| Calculated Molecular Weight | 44.2 kDa |
| Observed Molecular Weight | 50 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 100mM glycine, 10% Glycerol, pH 7.3. |
| Reconstitution | Not Applicable |
Background
Gastric Triacylglycerol Lipase (LIPF) belongs to the AB hydrolase superfamily. LIPF is an important lipase during the digestion of dietary lipids in cystic fibrosis. LIPF is involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. LIPF is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. LIPF acts distinct roles in neutral lipid metabolism.