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Recombinant Human GRB2 Protein (His Tag)
SKU: PKSH032512-50
Recombinant Human GRB2 Protein (His Tag)
| SKU # | PKSH032512 |
| Expression Host | E.coli |
Description
| Synonyms | ASH, Adapter Protein GRB2, GRB2, Growth Factor Receptor-Bound Protein 2, Protein Ash, SH2/SH3 Adapter GRB2 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Val217 |
| Accession | P62993 |
| Calculated Molecular Weight | 26.3 kDa |
| Observed Molecular Weight | 25-30 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 50mM NaCl, 0.05% Tween 80, pH8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.