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Recombinant Human IDO1/IDO Protein (His Tag)(Active)
SKU: PKSH032584-50
Recombinant Human IDO1/IDO Protein (His Tag)(Active)
| SKU # | PKSH032584 |
| Expression Host | E.coli |
Description
| Synonyms | 3-dioxygenase, 3-dioxygenase 1, IDO, IDO-1, IDO1, INDO, Indole 2, Indoleamine 2 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Gly403 |
| Accession | P14902 |
| Calculated Molecular Weight | 46.8 kDa |
| Observed Molecular Weight | 40-50 kDa |
| Tag | N-His |
| Bio-activity | Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is 5166. 667 pmol/min/µg pmol/min/μg. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Sodium Acetate, 150mM NaCl, 20% Glycerol, pH 4.5. |
| Reconstitution | Not Applicable |
Background
Indoleamine 2,3-dioxygenase (IDO) is a heme enzyme that initiates the oxidative degradation of the least abundant, essential amino acid, l-tryptophan, along the kynurenine pathway. This protein is normally expressed in the dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. IDO activity is associated with immunosuppression and immune attenuation. Several studies showed that IDO can contribute to immune escape when expressed directly in tumor cells or when expressed in immunosuppressive antigen presenting cells such as tolerogenic dendritic cells or tumor associated macrophages. IDO also is a promising therapeutic target for the treatment of cancer, chronic viral infections, and other diseases characterized by pathological immune suppression.