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Recombinant Human IGF-1/IGF1 Protein (aa 52-118)(Active)
SKU: PKSH032595-50
Recombinant Human IGF-1/IGF1 Protein (aa 52-118)(Active)
| SKU # | PKSH032595 |
| Expression Host | E.coli |
Description
| Synonyms | IBP1, IGF-I, IGF1, Insulin-Like Growth Factor I, MGF, Mechano Growth Factor, Somatomedin-C |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Thr52-Ala118 |
| Accession | P05019 |
| Calculated Molecular Weight | 7.3 kDa |
| Observed Molecular Weight | 9 kDa |
| Tag | None |
| Bio-activity | Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is 20-100 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.5 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids with 94% identity with mouse IGF1 and exhibits cross-species activity. IGF1 binds IGF-1R; IGF-2R; and the insulin receptor and plays a key role in cell cycle progression; cell proliferation and tumor progression. IGF1 expression is regulated by growth hormone.