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Recombinant Human IL-17A&IL-17F Heterodimer Protein (His Tag)(Active)
SKU: PKSH032619-50
Recombinant Human IL-17A&IL-17F Heterodimer Protein (His Tag)(Active)
| SKU # | PKSH032619 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Interleukin-17A, IL-17, IL-17A, IL17A, IL17 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gly24-Ala155&Arg31-Gln163 |
| Accession | Q16552&AAH70124.1 |
| Calculated Molecular Weight | 15.1&16 kDa |
| Observed Molecular Weight | 15-25 kDa |
| Tag | C-His |
| Bio-activity | Loaded Biotinylated Human IL-17RA-His-Avi on SA Biosensor, can bind Human IL-17A&17F-His with an affinity constant of 8.6 pM as determined in BLI assay. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The IL-17 family include IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F. The family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes.