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Recombinant Human IL15RA&IL15 Fusion Protein (Fc Tag)(Active)
SKU: PKSH032567-50
Recombinant Human IL15RA&IL15 Fusion Protein (Fc Tag)(Active)
| SKU # | PKSH032567 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | MGC, IL15RA, CD215, alpha, CD215 antigen, IL-15 receptor subunit alpha, IL-15RA, IL-15R-alpha, interleukin 15 receptor, interleukin-15 receptor subunit alpha, MGC104179, AA690181, I15RA, IL 15R alpha, Interleukin 15 receptor alpha, Interleukin 15 receptor subunit alpha, sIL-15 receptor subunit alpha, sIL-15RA, sIL-15R-alpha, Soluble interleukin 15 receptor subunit alpha, Soluble interleukin-15 receptor subunit alpha |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ile31-Asp96&Asn49-Ser162(Asn120Asp) |
| Accession | Q13261&P40933 |
| Calculated Molecular Weight | 46.9 kDa |
| Observed Molecular Weight | 50-60 kDa |
| Tag | C-Fc |
| Bio-activity | Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells.The ED50 for this effect is 5-20 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 8% Trehalose, 4% Mannitol, 0.02% Tween 80 (w/v), pH 7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
IL15RA is a high-affinity receptor for interleukin-15. Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide variety of Tand B cells and non-lymphoid cells.IL-15 is a cytokine that regulates T cell and natural killer cell activation and proliferation. IL-15 binds to the alpha subunit of the IL-15RA with high affinity. IL-15 also binds to the beta and gamma chains of the IL-2 receptor, but not the alpha subunit of the IL2 receptor. IL-15 is structurally and functionally related to IL-2. Both cytokines share some subunits of receptors, allowing them to compete for and negatively regulate each other's activity. The number of CD8+ memory T cells is controlled by a balance between IL-15 and IL-2. Despite their many overlapping functional properties, IL-2 and IL-15 are, in fact, quite distinct players in the immune system. IL-15 is constitutively expressed by a wide variety of cell types and tissues, including monocytes, macrophages and DCs.