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Recombinant Human IL1R2/CD121b Protein (His Tag)(Active)
SKU: PKSH031865-100
Recombinant Human IL1R2/CD121b Protein (His Tag)(Active)
| SKU # | PKSH031865 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | IL1R, Interleukin-1 receptor type, IL-1R, IL1R2, CD121b, CDw121b, IL-1R-2, IL-1RT-2, IL-1RT2, IL1R2c, IL1RB, CD121 antigen-like family member B, IL-1 type II receptor, IL-1R-beta, Interleukin-1 receptor beta, Interleukin-1 receptor type 2, Interleukin-1 receptor type I, IL-1R type II, IL-1RII |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Glu 343 |
| Accession | NP_004624.1 |
| Calculated Molecular Weight | 39.3 kDa |
| Observed Molecular Weight | 50-55 kDa |
| Tag | C-His |
| Bio-activity | 1. Measured by its ability to inhibit IL1β-dependent proliferation in D10.G4. 1 mouse helper T cells. The ED50 for this effect is typically 5. 5 μg/mL in the presence of 40 pg/mL of rhIL1β. 2. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500pg/mL IL1b. The EC50 for this effect is typically 20-80ng/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin 1 receptor; type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A); interleukin beta (IL1B); and interleukin 1 receptor; type I (IL1R1/IL1RA); and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses; and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified; designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling; whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains; a single transmembrane region; and a short cytoplasmic domain; is expressed in a variety of cell types including B lymphocytes; neutrophils; monocytes; large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.