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Recombinant Human Interleukin-18/IL-18 Protein
SKU: PKSH033626-100
Recombinant Human Interleukin-18/IL-18 Protein
| SKU # | PKSH033626 |
| Expression Host | E.coli |
Description
| Synonyms | MGC, IL1F, IL-1F, IL18, IGIF, IL-18, IL-1g, IL1F4, GIF, Iboctadekin, IFN-gamma-inducing factor, IL-1 gamma, IL-1F4, Interferon gamma-inducing factor, Interferon-gamma-inducing factor, Interleukin 18, Interleukin-1 gamma, Interleukin-18, MGC12320, IFN gamma inducing factor, IL 1 gamma, IL 18, IL 1g, IL1 gamma, IL18 protein, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interleukin 1 gamma, Interleukin 18 (interferon-gamma-inducing factor), Interleukin18 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Tyr37-Asp193 |
| Accession | Q14116 |
| Calculated Molecular Weight | 18.2 kDa |
| Observed Molecular Weight | 15 kDa |
| Tag | None |
| Bio-activity | Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is <6 ng/mL. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-18 is a secreted protein and it belongs to the IL-1 family. IL-18 is a proinflammatory cytokine and produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. After stimulation with IL-18; natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells.