Recombinant Human Interleukin-31/IL-31 Protein
SKU: PKSH032640-100
Recombinant Human Interleukin-31/IL-31 Protein
SKU # | PKSH032640 |
Expression Host | E.coli |
Description
Synonyms | Interleukin, IL31, IL-31, Interleukin-31, IL 31, Interleukin 31 |
Species | Human |
Expression Host | E.coli |
Sequence | Ser24-Thr164 |
Accession | Q6EBC2 |
Calculated Molecular Weight | 16.8 kDa |
Observed Molecular Weight | 18 kDa |
Tag | C-His |
Bio-activity | Measured by its ability to induce A549 cells proliferation. The ED50 for this effect is < 40 ng/mL |
Properties
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Interleukin 31 (IL-31) is a cytokine containing a four-helix bundle structure. It shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. Human IL-31 cDNA encodes a 164 amino acid precursor that contains a 23 amino acid signal peptide and a 141 amino acid mature protein. Human and mouse IL-31 share 24% sequence identity in the mature region. IL-31 is mainly associated with activated T cells and is preferentially expressed by type 2 helper T cells (Th2). IL-31 signals via a heterodimeric receptor complex composed of a gp130 related molecule termed IL-31RA (also GPL and GLMR) and an Oncostatin M receptor (OSM Rβ). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFNγ on monocytes. GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Studies have shown that IL31 induces severe pruritis (itching) and dermatitis in transgenic mice.