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Recombinant Human Interleukin-33/IL-33 Protein
SKU: PKSH033616-50
Recombinant Human Interleukin-33/IL-33 Protein
| SKU # | PKSH033616 |
| Expression Host | E.coli |
Description
| Synonyms | DVS, IL1F, Interleukin, C9orf, IL-1F, IL33, C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, DVS27 related protein, IL-1F11, IL-33, Interleukin-1 family member 11, Interleukin-33, Nuclear factor from high endothelial venules, NFHEV, CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, IL 1F11, IL 33, Interleukin 1 family member 11, Interleukin 33, INTERLEUKIN 33 NFHEV, Interleukin 33 precursor, Interleukin33, Interleukin-33 (109-270), NF HEV, Nuclear factor for high endothelial venules, OTTHUMP00000021041, RP11 575C20.2 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ser112-Thr270 |
| Accession | O95760 |
| Calculated Molecular Weight | 18.1 kDa |
| Observed Molecular Weight | 18 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.01 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils; Peyers's patches; and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus; and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment; corresponding to mature IL-33; binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2; activates NF-kappaB and MAP kinases; and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. In vivo; IL-33 induces the expression of IL-4; IL-5; and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.