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Recombinant Human Interleukin-4/IL-4 Protein (Active)
SKU: PKSH032650-100
Recombinant Human Interleukin-4/IL-4 Protein (Active)
| SKU # | PKSH032650 |
| Expression Host | E.coli |
Description
| Synonyms | Interleukin, B-cell growth factor, BSF, IL4, BCGF-1, BCGF1, BSF-1, BSF1, IL-4, B-cell growth factor 1, B-cell IgG differentiation factor, B-cell stimulatory factor 1, Binetrakin, IGG1 induction factor, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra |
| Species | Human |
| Expression Host | E.coli |
| Sequence | His25-Ser153 |
| Accession | P05112 |
| Calculated Molecular Weight | 15.9 kDa |
| Observed Molecular Weight | 12 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.2 ng/mL. The specific activity of recombinant human IL-4 is approximately >2.8 x 107 IU/mg. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation; survival and gene expression. IL-4 is produced by mast cells; T cells; and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells; characterized by their cytokine-secretion profile that includes secretion of IL-4; IL-5; IL-6; IL-10; and IL-13; which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.