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Recombinant Human KI67 protein(His tag)
SKU: PDEH100099-100
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Recombinant Human KI67 protein(His tag)
| SKU # | PDEH100099 |
| Expression Host | E. coli |
Description
| Synonyms | Antigen identified by monoclonal antibody Ki-67;Antigen KI-67;Antigen Ki67 |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Lys1001-Pro1112 |
| Accession | P46013 |
| Mol_Mass | 12.4 kDa |
| AP_Mol_Mass | 13 kDa |
| Tag | N-His |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly.Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface.Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.Binds DNA, with a preference for supercoiled DNA and AT-rich DNA.Does not contribute to the internal structure of mitotic chromosomes.May play a role in chromatin organization.It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).