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Recombinant Human Mesothelin/MSLN Protein (His & Avi Tag)
SKU: PKSH033798-100
Recombinant Human Mesothelin/MSLN Protein (His & Avi Tag)
| SKU # | PKSH033798 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CAK1, CAK1 antigen, MPF, MSLN, Megakaryocyte potentiating factor, Pre-pro-megakaryocyte-potentiating factor, SMR, mesothelin, soluble MPF mesothelin related protein |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Glu296-Ser598 |
| Accession | AAH09272.1 |
| Calculated Molecular Weight | 36.7 kDa |
| Observed Molecular Weight | 38-60 kDa |
| Tag | C-His-Avi |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Mesothelin is a cell surface glycoprotein whose expression is limited to mesothelial cells of the serosa (pleura, pericardium, and peritoneum) and epithelial cells of the trachea, tonsils, fallopian tube, and kidneys. Mesothelin plays an important role in cell survival, proliferation, migration, invasion, tumor progression, and resistance to chemotherapy. The overexpression of mesothelin can activate NF-κB and signal transducer and activator of transcription 3 (Stat3), inhibit apoptotic signaling and TNF-α-induced apoptosis, and accelerate the G1–S transition. Mesothelin is also found overexpressed in various cancers, including malignant mesothelioma, pancreatic or ovarian carcinoma, sarcomas and in some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues, mesothelin has been reported as an ideal tumor-associated marker for the development of targeted therapy.