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Recombinant Human NACHRA5/CHRNA5 Protein (His Tag)
SKU: PKSH033582-50
Recombinant Human NACHRA5/CHRNA5 Protein (His Tag)
| SKU # | PKSH033582 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CHRNA5, NACHRA5, Neuronal Acetylcholine Receptor Subunit Alpha-5 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Arg23-Thr254 |
| Accession | P30532 |
| Calculated Molecular Weight | 27.6 kDa |
| Observed Molecular Weight | 35-47 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Neuronal Acetylcholine Receptor Subunit α-5 (NACHRA5) is a member of the ligand-gated ion channel family. Neuronal AChR is composed of two different type of subunits: α and non-α. When NACHRA5 binds to acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits, leading to the opening of an ion-conducting channel across the plasma membrane. Genetic variations in NACHRA5 have been related to susceptibility to smoking-related behavioral traits and lung cancer, contributing to the smoking quantitative trait locus 3.