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Recombinant Human Polyubiquitin-B/UBB Protein (GST&His Tag)
SKU: PKSH032916-50
Recombinant Human Polyubiquitin-B/UBB Protein (GST&His Tag)
| SKU # | PKSH032916 |
| Expression Host | E.coli |
Description
| Synonyms | Polyubiquitin-B, UBB |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 153-Gly228 |
| Accession | P0CG47 |
| Calculated Molecular Weight | 9.4 kDa |
| Observed Molecular Weight | 7 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Polyubiquitin-B(UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.