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Recombinant Human PRKD2/PKD2 Protein (His & GST Tag)(Active)
SKU: PKSH030353-50
Recombinant Human PRKD2/PKD2 Protein (His & GST Tag)(Active)
| SKU # | PKSH030353 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | HSPC187, PKD2, nPKC-D2 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-Leu 878 |
| Accession | NP_057541.2 |
| Calculated Molecular Weight | 124 kDa |
| Observed Molecular Weight | 120 kDa |
| Tag | N-His-GST |
| Bio-activity | The specific activity was determined to be > 30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate. |
Properties
| Purity | > 82 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0 |
| Reconstitution | Not Applicable |
Background
Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to theprotein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains onePH domain, twophorbol-ester/DAG-type zinc fingers and oneprotein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.