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Recombinant Human RIDA/HRSP12 Protein (His Tag)
SKU: PKSH032526-50
Recombinant Human RIDA/HRSP12 Protein (His Tag)
| SKU # | PKSH032526 |
| Expression Host | E.coli |
Description
| Synonyms | 14.5 kDa Translational Inhibitor Protein, HRSP12, Heat-Responsive Protein 12, PSP, Ribonuclease UK114, UK114 Antigen Homolog, p14.5 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Leu137 |
| Accession | P52758 |
| Calculated Molecular Weight | 16.7 kDa |
| Observed Molecular Weight | 16 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Heat-Responsive Protein 12 (HRSP12) is an endoribonuclease that belongs to the Rut family. HRSP12 is found mainly in the human adult kidney and liver and is responsible for inhibiting protein translation by cleaving mRNA. HRSP12 only cleaves phosphodiester bonds in single-stranded RNA and inhibits cell-free protein synthesis. The levels of both mRNA and protein are markedly reduced in heptatocellular tumors and in human hepatoma cell lines compared with normal liver tissues. Moreover the levels of HRSP12 are different depending on the grade of the tumor. This had led to the suggestion that HRSP12 may be an important biomarker for heptatic carcinoma.