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Recombinant Human RPE Protein (E.coli, His Tag)
SKU: PKSH033347-50
Recombinant Human RPE Protein (E.coli, His Tag)
| SKU # | PKSH033347 |
| Expression Host | E.coli |
Description
| Synonyms | HUSSY-17, RPE, RPE2-1, Ribulose-5-Phosphate-3-Epimerase, Ribulose-Phosphate 3-Epimerase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Arg228 |
| Accession | Q96AT9-1 |
| Calculated Molecular Weight | 25.9 kDa |
| Observed Molecular Weight | 28 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 6.2. |
| Reconstitution | Not Applicable |
Background
Ribulose-Phosphate 3-Epimerase (RPE) is a member of the Ribulose-Phosphate 3-Epimerase family. RPE exists as a homodimer and catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. RPE binds one divalent metal cation per subunit and contains tightly bound Fe2+ when produced in E. coli, but the physiological cofactor may be Co2+, Mn2+ or Zn2+. It has been shown that RPE participates in 3 metabolic pathways: pentose phosphate pathway, pentose and glucuronate interconversions, and carbon fixation.