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Recombinant Human SCF/c-Kit Ligand Protein (His Tag)(Active)
SKU: PKSH033081-50
Recombinant Human SCF/c-Kit Ligand Protein (His Tag)(Active)
| SKU # | PKSH033081 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | KITLG, Kit Ligand, MGF, Mast Cell Growth Factor, SCF, Stem Cell Factor, c-Kit ligand |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Glu26-His214 |
| Accession | P21583 |
| Calculated Molecular Weight | 22.0 kDa |
| Observed Molecular Weight | 30-50 kDa |
| Tag | C-His |
| Bio-activity | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-10 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.