Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated
SKU: 1234
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Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated
SKU: PKSH033890-100
Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated
| SKU # | PKSH033890 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD33 antigen-like 3, CD33L3, Sialic acid-binding Ig-like lectin 15, Siglec-15 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Phe20-Thr263 |
| Accession | Q6ZMC9 |
| Calculated Molecular Weight | 55.3 kDa |
| Observed Molecular Weight | 58-75 kDa |
| Tag | C-Fc-Avi |
| Bio-activity | Loaded Biotinylated Human Siglec-15-Fc on AHC Biosensor, can bind Anti-Human Siglec15 mAb-mFc with an affinity constant of 0.13 nM as determined in BLI assay. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.