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Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (Active)
SKU: PKSH030315-50
Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (Active)
| SKU # | PKSH030315 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | SPHK, SPHK1, SPK |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-Leu 384 |
| Accession | Q9NYA1-1 |
| Calculated Molecular Weight | 42.7 kDa |
| Observed Molecular Weight | 46 kDa |
| Tag | None |
| Bio-activity | The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate. |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0 |
| Reconstitution | Not Applicable |
Background
SPHK1; also known as sphingosine Kinase 1; catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation; survival; migration; cytoskeletal organization; and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory; antiapoptotic; and immune processes.