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Recombinant Human SRC Kinase/c-SRC Protein (His & GST Tag)(Active)
SKU: PKSH030385-50
Recombinant Human SRC Kinase/c-SRC Protein (His & GST Tag)(Active)
| SKU # | PKSH030385 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | ASV, SRC1, c-SRC, p60-Src |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-Leu 536 |
| Accession | P12931-1 |
| Calculated Molecular Weight | 87.7 kDa |
| Observed Molecular Weight | 81 kDa |
| Tag | N-His-GST |
| Bio-activity | The specific activity was determined to be > 80 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT |
| Reconstitution | Not Applicable |
Background
Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.