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Recombinant Human STUB1 Protein
SKU: PKSH032367-50
Recombinant Human STUB1 Protein
| SKU # | PKSH032367 |
| Expression Host | E.coli |
Description
| Synonyms | Antigen NY-CO-7, CHIP, CLL-Associated Antigen KW-8, Carboxy Terminus of Hsp70-Interacting Protein, E3 Ubiquitin-Protein Ligase CHIP, STIP1 Homology and U Box-Containing Protein 1, STUB1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Tyr303 |
| Accession | Q9UNE7 |
| Calculated Molecular Weight | 34.9 kDa |
| Observed Molecular Weight | 33 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH7.4. |
| Reconstitution | Not Applicable |
Background
E3 Ubiquitin-Protein Ligase CHIP is a cytoplasmic protein. CHIP is highly expressed in skeletal muscle, heart, pancreas, brain and placenta. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain; lead to in client substrate ubiquitylation and degradation by the proteasome. CHIP targets misfolded chaperone substrates towards proteasomal degradation. CHIP mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. CHIP plays a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. It also may regulate the receptor stability and activity through proteasomal degradation.