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Recombinant Human Survivin/BIRC5 Protein
SKU: PKSH032109-50
Recombinant Human Survivin/BIRC5 Protein
| SKU # | PKSH032109 |
| Expression Host | E.coli |
Description
| Synonyms | API4, Apoptosis Inhibitor 4, Apoptosis Inhibitor Survivin, BIRC5, Baculoviral IAP Repeat-Containing Protein 5, IAP4 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Asp142 |
| Accession | O15392 |
| Calculated Molecular Weight | 16.4 kDa |
| Observed Molecular Weight | 16-18 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Baculoviral IAP Repeat-Containing Protein 5 (BIRC5) belongs to the IAP family. BIRC5 exists as a homodimer in the apo state and as a monomer in the CPC-bound state. BIRC5 contains one BIR repeat and is expressed only in fetal kidney and liver, and to lesser extent, lung and brain. BIRC5 functions as a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. BIRC5 is also a component of a chromosome passage protein complex (CPC), which is essential for chromosome alignment and segregation during mitosis and cytokinesis. BIRC5 acts as an important regulator of the localization of this complex.