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Recombinant Human TCN2 Protein (His Tag)(Active)
SKU: PKSH031522-50
Recombinant Human TCN2 Protein (His Tag)(Active)
| SKU # | PKSH031522 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | D22S676, D22S750, TC-2, TC2, TCII, Transcobalamin-II |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Trp 427 |
| Accession | NP_000346.2 |
| Calculated Molecular Weight | 46.7 kDa |
| Observed Molecular Weight | 43 kDa |
| Tag | C-His |
| Bio-activity | Immobilized human TCN2-His at 10μg/mL (100μL/well) can bind biotinylated mouse CD320-His. The EC50 of biotinylated mouse CD320-His is 18-42 ng/mL. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.