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Recombinant Human TFIIB/GTF2B Protein (GST Tag)
SKU: PKSH033135-50
Recombinant Human TFIIB/GTF2B Protein (GST Tag)
| SKU # | PKSH033135 |
| Expression Host | E.coli |
Description
| Synonyms | GTF2B, General Transcription Factor TFIIB, S300-II, TF2B, TFIIB, Transcription Initiation Factor IIB |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Leu316 |
| Accession | Q00403 |
| Calculated Molecular Weight | 61.6 kDa |
| Observed Molecular Weight | 62 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.