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Recombinant Human Thioredoxin/TXN Protein (His Tag)(Active)
SKU: PKSH033108-50
Recombinant Human Thioredoxin/TXN Protein (His Tag)(Active)
| SKU # | PKSH033108 |
| Expression Host | E.coli |
Description
| Synonyms | ADF, ATL-Derived Factor, SASP, Surface-Associated Sulphydryl Protein, TRDX, TRX, TRX1, TXN, Thioredoxin, Trx |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Val105 |
| Accession | P10599 |
| Calculated Molecular Weight | 13.9 kDa |
| Observed Molecular Weight | 14 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 1mM EDTA, 2mM DTT, pH 7.2. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Thioredoxin (TXN) is a member of the Thioredoxin family. Thioredoxin exists as a disulfide-linked homodimer and contains one Thioredoxin domain. Thioredoxin is up-regulated by ionizing radiation. Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin also plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide.