Recombinant Human Tie2/CD202b Protein (His & Fc Tag)(Active)
SKU: PKSH031473-200
Recombinant Human Tie2/CD202b Protein (His & Fc Tag)(Active)
SKU # | PKSH031473 |
Expression Host | HEK293 Cells |
Description
Synonyms | CD202B, TIE-2, TIE2, VMCM, VMCM1 |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Met 1-Lys 745 |
Accession | NP_000450.2 |
Calculated Molecular Weight | 108.5 kDa |
Observed Molecular Weight | 125-135 kDa |
Tag | C-His-Fc |
Bio-activity | Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml. |
Properties
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature. vasculature.