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Recombinant Human TRAPPC8 protein (His tag)
SKU: PDEH100255-100
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Recombinant Human TRAPPC8 protein (His tag)
| SKU # | PDEH100255 |
| Expression Host | E. coli |
Description
| Synonyms | Trafficking protein particle complex subunit 8;TRAPPC8;Protein TRS85 homolog;KIAA1012 |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Gly 100-Gln 250 |
| Accession | Q9Y2L5-1 |
| Mol_Mass | 16.5 kDa |
| AP_Mol_Mass | 20 kDa |
| Tag | N-His & C-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
The transport protein particle (TRAPP) complex was initially identified as a tethering factor for COPII vesicle. Subsequently, three forms (TRAPPI, II, and III) have been found and TRAPPIII has been reported to serve as a regulator in autophagy. This study investigates a new role of mammalian TRAPPIII in ciliogenesis. We found a ciliopathy protein, oral-facial-digital syndrome 1 (OFD1), interacting with the TRAPPIII-specific subunits TRAPPC8 and TRAPPC12. TRAPPC8 is necessary for the association of OFD1 with pericentriolar material 1 (PCM1).