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Recombinant Human Ube2H Protein
SKU: PKSH030772-100
Recombinant Human Ube2H Protein
| SKU # | PKSH030772 |
| Expression Host | E.coli |
Description
| Synonyms | E2-20K, GID3, UBC8, UBCH, UBCH2, UBE2H, UbcH2, Ubiquitin Carrier Protein H, Ubiquitin-Conjugating Enzyme E2 H, Ubiquitin-Conjugating Enzyme E2-20K, Ubiquitin-Protein Ligase H |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Leu 183 |
| Accession | P62256 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 93 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, 10% glycerol, 2mM DTT, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.