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Recombinant Human VAP-1/AOC3 Protein (His Tag)
SKU: PKSH032744-50
Recombinant Human VAP-1/AOC3 Protein (His Tag)
| SKU # | PKSH032744 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, Semicarbazide-sensitive amine oxidase, VAP-1, VAP1, Vascular adhesion protein 1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Arg28-Asn763 |
| Accession | Q16853 |
| Calculated Molecular Weight | 82.6 kDa |
| Observed Molecular Weight | 90-110 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Vascular adhesion protein-1(VAP-1) is a copper amine oxidase with a topaquinone cofactor.VAP-1 is a type II integral membrane protein; but a soluble form of the enzyme is present in human serum; and its level increases in diabetes and some inflammatory liver diseases. VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine; benzylamine; and aminoacetone in a reaction that produces an aldehyde; ammonia; and H2O2. VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored. The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues. VAP-1 mediated adhesion is involved in the process of leukocyte extravasation; an important feature of inflammatory responses. VAP-1 is considered to be a therapeutic target for diabetes; oxidative stress; and inflammatory diseases.