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Recombinant Human Vitamin D-Binding Protein/GC Protein (His tag)
SKU: PDMH100111-100
Recombinant Human Vitamin D-Binding Protein/GC Protein (His tag)
| SKU # | PDMH100111 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | DBP, GC, Gc-Globulin, Group-Specific Component, VDB, Vitamin D-Binding Protein |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met1-Leu474 |
| Accession | P02774 |
| Calculated Molecular Weight | 52.0 kDa |
| Observed Molecular Weight | 57 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background
Vitamin D-Binding Protein (DBP) is a member of the ALB/AFP/VDB family. DBP is a secreted protein and contains three albumin domains. The primary structure contains 28 cysteine residues forming multiple disulfide bonds. DBP acts as a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. DBP binds to vitamin D and its plasma metabolites and transports them to target tissues. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.