Total Price: $0.00
Contact Information
Recombinant Vaccinia Virus B18R / B19R Protein (His Tag)
SKU: PKSV030257-100
Recombinant Vaccinia Virus B18R / B19R Protein (His Tag)
| SKU # | PKSV030257 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | Vaccinia Virus B18R;B19R |
| Species | Vaccinia Virus |
| Expression_host | Baculovirus-Insect Cells |
| Sequence | Met1-Glu351 |
| Accession | P25213 |
| Mol_Mass | 39.8 kDa |
| AP_Mol_Mass | 46 kDa |
| Tag | C-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
B18R is a type I interferon (IFN)-binding protein, which is encoded by the B18R open reading frame in the WR (WesternReserve)strain of vaccinia virus. Itis alsoknownasB19RintheCopenhagenstrainofVaccinia. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. It has been shown that secreted B18R binds to uninfected and infected cells. It presents at the cell surface and protects cells from the antiviral state induced by IFN-alpha and IFN-beta. Binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.