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SARS-CoV-2 Neutralization Antibody ELISA Kit
SKU: E-EL-E606
SARS-CoV-2 Neutralization Antibody ELISA Kit
Product Details
Properties
| Assay Type | One step Competitive-ELISA |
| Format | 96T |
| Assay time | 1.5h |
| Sample type | serum and plasma |
| Sample volume | 50 μL |
| Storage | 2-8℃ |
| Interpretation | Qualitative |
| Application | This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma. |
| Reproducibility | Both intra-CV and inter-CV are < 15% |
| Synonyms | COVID 19 Neutralization Antibody, Anti-RBD, RBD Antibody, S1RBD Antibody |
Test Principle
This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. _x000D_ The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.
Kit Components and Storage
The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the
reagents according to the conditions on the next page.
| Item | Specifications | Storage |
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips | 2-8℃, 6 months |
| 48T: 8 wells ×6 strips | ||
| Positive Control | 96T: 2 vials | |
| 48T: 1 vial | ||
| Negative Control | 96T: 2 vials | |
| 48T: 1 vial | ||
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | -20℃ (Protect from light), 6 months |
| 48T: 1 vial, 60 μL | ||
| Sample & Control Diluent | 2 vial, 20 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer(25×) | 1 vial, 30 mL | |
| Stop Solution | 1 vial, 10 mL | |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) , 6 months |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
Assay Procedures
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1. Add 50µL each pre-treated Samples and Controls. Immediately add 50µL of HRP-RBD working solution. Incubate for 60 min at 37°C. |
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2. Aspirate and wash the plate for 3 times. |
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3. Add 90µL of Substrate Reagent. Incubate for about 15 min at 37°C. |
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4. Add 50µL of Stop Solution. |
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5. Read the plate at 450nm immediately. Calculation of the results |