Thank you!

Your quote has been successfully submitted!

For products requiring additional information, our team will contact you within 1 business day

Failed

There was an error submitting your quote. Please try again.

Sucrose Colorimetric Assay Kit– MSE Supplies LLC

Free Shipping on MSE PRO Online Orders of $500 or More! U.S. Orders Only * Offer Excludes Hazmat Shipments *

Menu

Sucrose Colorimetric Assay Kit

SKU: E-BC-K161-S-100

To better serve you, we would like to discuss your specific requirement.
Please Contact Us for a quote.

Sucrose Colorimetric Assay Kit

SKU # E-BC-K161-S
Detection Instrument Spectrophotometer (290 nm)
Detection method Colorimetric method


Product Details

Properties

Sample Type Plant tissue
Sensitivity 0.32 μmol/mL
Detection Range 0.32-70 μmol/mL
Detection Method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 8.500% | Average intra-assay CV: 3.400%
Other instruments required Micropipettor, Vortex mixer, 100℃ Water bath
Other reagents required PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months


Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.32-70 μmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Green pepper tissue homogenization 1
10% Epipremnum aureum tissue homogenization 1
10% Cucumber tissue homogenization 1

 

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

 

Detection Principle

Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.

 

Kit Components & Storage

Item Component Size 1
(50 Assays)
Size 2
(100 Assays)
Storage
Reagent 1 Hydrolysate Solution 60 mL × 2 vials 60 mL × 4 vials 2-8°C, 12 months
Reagent 2 100 μmol/mL Sucrose Standard 1 mL × 1 vial 1 mL × 1 vial 2-8°C, 12 months

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three cucumber tissue samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/mL) 8.50 34.80 58.00
%CV 3.7 3.4 3.1


Inter-assay Precision

Three rat liver tissue samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/mL) 8.50 34.80 58.00
%CV 8.3 8.1 9.1

 

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 102%.

Standard 1 Standard 2 Standard 3
Expected Conc. (μmol/mL) 12.6 45.5 60
Observed Conc. (μmol/mL) 13.4 44.1 61.8
Recovery rate (%) 106 97 103

 

Sensitivity

The analytical sensitivity of the assay is 0.32 μmol/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

 

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only.

Concentration (mmol/L) 0 10 20 30 40 50 70
Average OD 0.007 0.296 0.600 0.921 1.220 1.547 2.071
Absoluted OD 0 0.289 0.593 0.914 1.213 1.540 2.064