Total Bilirubin (TBIL) Colorimetric Assay Kit

Total Bilirubin (TBIL) Colorimetric Assay Kit

Product Details

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BB Liu et al investigate the function of SHCZ in ameliorating liver injury. Total bilirubin (TBIL) in rat liver was determined using TBIL colorimetric assay kit (E-BC-K760-M).

The concentration of TBA was significantly changed in each group. (**P<0.01 vs Normal, #P<0.05, ##P<0.01 vs Model, △P<0.05, △△△P<0.001 vs High-dose)

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.7-50 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Note: The diluent is normal saline (0.9% NaCl).

Detection Principle

Bilirubin is one of the important components of bile. It is the degradation product of hemoglobin in various heme proteins under the action of a series of enzymes. It is important to the digestion and absorption of lipids and the formation of yellow distemper. Bilirubin comes in two forms: water-soluble and water-insoluble. Bilirubin has powerful antioxidant, anti-inflammatory and autoimmune properties. The concentration of bilirubin in human body is related to sex, drug intake, age and so on. Low serum bilirubin is directly related to diabetes, metabolic syndrome, cardiovascular disease and other pathological states. However, high bilirubin is indicative of hemolysis, jaundice, Gilbert syndrome, hepatitis, drug toxicity, and possible bile duct obstruction.

Kit Components & Storage

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 96%.

Sensitivity

The analytical sensitivity of the assay is 0.7 μmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the
actual assay performance (e.g. operator, pipetting technique or temperature effects),
so the standard curve and data are provided as below for reference only: