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Total Protein Extraction Assay Kit
SKU: E-BC-E002-100
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Total Protein Extraction Assay Kit
| SKU # | E-BC-E002 |
Product Details
Properties
| Sample type | Animal tissues, cells |
| Other instruments required | High-speed freezing centrifuge, 5mL Glass homogenizer |
| Other reagents required | Double distilled water, PBS(0.01 M, pH=7.4) |
| Storage | Reagent 1: 2-8℃; Others: -20℃ |
| Valid period | 12 months |
Detection Principle
Cell and tissue samples are treated with lysates containing protease inhibitors and phosphatase inhibitors to prevent the enzymes in the sample from being released to hydrolyze or dephpsphprylate protein due to the disruption of the membrane system.
Kit Components & Storage
| Item | Component | Size 1 (50 assays) |
Size 2 (100 assays) |
Storage |
| Reagent 1 | Lysis Buffer | 60 mL × 1 vial | 60 mL × 2 vials | 2-8℃, 12 months |
| Reagent 2 | Phosphatase Inhibitor | 0.6 mL × 1 vial | 1.2 mL × 1 vial | -20°C, 12 months, shading light |
| Reagent 3 | Protease Inhibitor | 0.6 mL × 1 vial | 1.2 mL × 1 vial | -20°C, 12 months, shading light |
| Reagent 4 | Phenylmethylsulfonyl Fluoride |
0.6 mL × 1 vial | 1.2 mL × 1 vial | -20°C, 12 months, shading light |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.