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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)
SKU: E-BC-K238-96
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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)
| SKU # | E-BC-K238 |
| Detection Instrument | Microplate reader (510 nm), Biochemistry analyzer (510 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | TG |
| Sample Type | Serum, plasma, cells, culture supernatant |
| Detection Range | 0-9.04 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 20 min |
| Storage | 2-8℃ |
| Valid period | 12 months |
Images
N A M Omar et al investigate the effect of high intakes of fructose and galactose, with or without added fructooligosaccharides (FOS), on metabolism factors, inflammation, and gut integrity markers in rats. Triglyceride (TG) level in rat plasma was determined using TG colorimetric assay kit (E-BC-K238).
Plasma TG level shows no significant differences with different diets (P<0.05).
Detection Principle
The color depth of the generated quinones is directly proportional to the triglyceride content. The absorbance values of the standard tube and the sample tube are measured respectively, and the triglyceride content in the sample can be calculated.
Kit Components & Storage
| Item | Component | Size (96 T) | Storage |
| Reagent 1 | Enzyme Working Solution |
25 mL × 1 vial | 2-8℃, 12 months, shading light |
| Reagent 2 | 2.26 mmol/L Glycerinum Standard | 0.1 mL × 1 vial | 2-8℃, 12 months, shading light |
| Microplate | 96 wells | No requirement | |
| Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 1.50 | 5.00 | 7.50 |
| %CV | 4.4 | 3.5 | 4.4 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 1.50 | 5.00 | 7.50 |
| %CV | 9.0 | 9.3 | 9.4 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 105%.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Expected Conc. (mmol/L) | 0.35 | 5.3 | 9.2 |
| Observed Conc. (mmol/L) | 0.4 | 5.7 | 9.4 |
| Recovery rate (%) | 105 | 108 | 102 |
Sensitivity
The analytical sensitivity of the assay is 0.14 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.