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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Meth– MSE Supplies LLC

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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

SKU: E-BC-K261-S-100

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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

SKU # E-BC-K261-M
Detection Instrument Spectrophotometer (510 nm)
Detection method Colorimetric method


Product Details

Properties

Synonyms TG
Sample type Serum, plasma, tissue
Sensitivity 0.19 mmol/L
Detection range 0.19-8.0 mmol/L
Detection Method Colorimetric method
Assay type Quantitative
Assay time 50min
Precision Average inter-assay CV: 6.700% | Average intra-assay CV: 2.400%
Storage 2-8℃
Valid period 12 months


Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.19-8.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Mouse serum 1
Rat plasma 1
10% Rat liver tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Mouse brain tissue homogenate 1

 

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples; The diluent is anhydrous ethanol for tissue samples.

 

Detection Principle

The color depth of the generated quinones is directly proportional to the triglyceride content. The absorbance values of the standard tube and the sample tube are measured respectively, and the triglyceride content in the sample can be calculated.


Kit Components & Storage

Item Component Size (96 T) Storage
Reagent 1 Enzyme Working Solution 100 mL × 1 vial 2-8℃, 12 months shading light
Reagent 2 2.26 mmol/L Glycerinum Standard 0.1 mL × 1 vial 2-8℃, 12 months shading light

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters Sample 1 Sample 2 Sample 3
Mean (mmol/L) 0.66 2.50 6.40
%CV 2.7 2.3 2.2


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (mmol/L) 0.66 2.50 6.40
%CV 6.2 6.8 7.1


Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 94%.

Parameters Sample 1 Sample 2 Sample 3
Expected Conc. (mmol/L) 0.85 3.4 7.2
Observed Conc. (mmol/L) 0.8 3.2 6.6
Recovery rate (%) 98 93 91


Sensitivity

The analytical sensitivity of the assay is 0.019 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.


Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration
(mmol/L)
0 1 2 4 5 6 8
Average OD 0.075 0.140 0.193 0.301 0.508 0.700 0.922
Absoluted OD 0.000 0.065 0.118 0.226 0.433 0.625 0.847