Thank you!

Your quote has been successfully submitted!

For products requiring additional information, our team will contact you within 1 business day

Failed

There was an error submitting your quote. Please try again.

Uric Acid (UA) Colorimetric Assay Kit– MSE Supplies LLC

Free Shipping on MSE PRO Online Orders of $500 or More! U.S. Orders Only * Offer Excludes Hazmat Shipments *

Menu

Uric Acid (UA) Colorimetric Assay Kit

SKU: E-BC-K016-S-100

To better serve you, we would like to discuss your specific requirement.
Please Contact Us for a quote.

Uric Acid (UA) Colorimetric Assay Kit

SKU # E-BC-K016-S
Detection Instrument Spectrophotometer(690 nm)
Detection Method Colorimetric method


Product Details

Properties

Synonyms UA
Sample Type Serum, plasma, urine
Sensitivity 0.58 mg/L
Detection Range 0.58-100 mg/L
Detection Method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 2.600% | Average intra-assay CV: 1.800%
Other instruments required Vortex mixer, Micropipettor, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

 

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human urine 8-10
Human serum 1
Dog serum 1
Rat serum 1
Mouse serum 1-2
Porcine serum 1

 

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

 

Detection Principle

Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid.

 

Kit Components & Storage

Item Component Size 1
(50 assays)
Size 2
(100 assays)
Storage
Reagent 1 1 g/L Uric Acid Standard 1 mL ×1 vial 1 mL ×1 vial 2-8℃, 12 months
Reagent 2 Protein Precipitator 60 mL ×2 vials 60 mL × 4 vials 2-8℃, 12 months
Reagent 3 Alkali Reagent 30 mL ×1 vial 60 mL × 1 vial 2-8℃, 12 months
Reagent 4 Phosphotungstic Acid Reagent 30 mL ×1 vial 60 mL × 1 vial 2-8℃, 12 months shading light

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters Sample 1 Sample 2 Sample 3
Mean (mg/L) 1.50 25.00 75.00
%CV 2.3 1.9 1.2


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (mg/L) 1.50 25.00 75.00
%CV 2.4 2.7 2.7


Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 105%.


Standard 1 Standard 2 Standard 3
Expected Conc. (mg/L) 20 35 65
Observed Conc. (mg/L) 20.4 37.1 69.6
Recovery rate (%) 102 106 107


Sensitivity

The analytical sensitivity of the assay is 0.58 mg/L UA. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.


Standard Curve:

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (mg/L) 0 10 40 60 80 100
Average OD 0.001 0.017 0.083 0.138 0.188 0.235
Absoluted OD 0 0.016 0.082 0.137 0.187 0.234