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Water-soluble Biotin Labeling Kit (50 KD Filtration Tube)
SKU: E-LK-B007C-10
Water-soluble Biotin Labeling Kit (50 KD Filtration Tube)
Introduction
Elabscience® Water-soluble Biotin Labeling Kit provides all the reagents required for labeling, which can label proteins containing primary amino-group (-NH2) molecules simply and effectively.
Technical Information
| Technical Information | Elabscience® Water-soluble Biotin Labeling Kit provides all the reagents required for labeling, which can label antibodies and other proteins containing primary amino-group (-NH2) molecules simply and effectively. |
| Applications | Protein labeling/Antibody labeling (50 KD Filtration tube) |
| Principle |  |
| Labeling group | Sulfo-NHS-Biotin |
Characteristic
- Fast: The whole process takes only 90 min.
- Convenient: The Sulfo-NHS-Biotin has been activated and can be used directly. Filtration tube desalts without dialysis.
- Flexible use: It can be used for both micro-labeling and large-scale labeling, and can label 0.1-1 mg proteins each time.
- Water solubility: Sulfo-NHS-Biotin in this kit is water soluble, which can be used to label proteins in aqueous buffer at a high concentration or molar ratio, while reducing the nonspecific binding of biotin to some proteins.
Materials Not Supplied
1. Pipettor and tips (0.5-10μL, 2-20μL, 20-200μL, 200-1000μL).
2. 37°C incubator.
3. Centrifuge (centrifugal force up to 12,000>g).
4. Vortex mixer.
Shipping & Storage Information
| Storage | 2~8°C/-20°C, shading light |
| Shipping | Ice bag |
| Expiration date | 12 months |
Notes
- Please select the appropriate kit according to the molecular weight of the protein to be labeled. The kit provides a 50 KD Filtration tube.
- Sulfo-NHS-Biotin is susceptible to moisture hydrolysis failure, and should be stored at -20°C or -80°C with the desiccant. In order to prevent water vapor from condensing into the Sulfo-NHS-Biotin, it is necessary to equilibrate the Sulfo-NHS-Biotin to room before the experiment.
- The kit can also be used to label other proteins containing free amino groups. The specific labeling ratio is determined according to the number of available amino groups in the marker or set different molar ratios for labeling.
- The optimal molecular ratio of the NHS-Biotin and protein (150KD) recommended for labeling with this kit is 20:1, which can ensure that the NHS-Biotin can label the protein, but it cannot ensure the best experimental results. The optimal labeling ratio may vary according to the difference of protein, and the user can optimize according to the actual situation.