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Anti-MBP Nanobody Affinity Agarose, 2mL
SKU: EA-IP-006N
Anti-MBP Nanobody Affinity Agarose, 2mL
Background
MBP (Maltose Binding Protein) is a commonly used affinity tag, which can increase the expression level and solubility of the target protein. Anti-MBP affinity gel is made by covalently coupling high-quality MBP nanobody with agarose gel. Since the nanobody only contains the variable region of antibody molecules, there will be no signal interference from the heavy and light chains of antibody during immuno (co) precipitation. It has the characteristics of high binding capacity of protein, high specificity and stability, and can be used for immuno (co) precipitation of MBP tagged fusion protein.
Performance Index
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Scope of application |
Immuno (co) precipitation of MBP tag fusion protein. The MBP tag can be located at the N-terminal and C-terminal of the protein, such as the N-terminal MBP fusion protein (MBP–Protein) and the C-terminal MBP fusion protein (Protein-MBP). |
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Antibody Properties |
Anti-MBP Nanobody. |
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Gel properties |
Agarose gel granules, average size 50 μm. |
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Binding capacity |
1mL Sepharose 4B agarose granules are covalently coupled with 6mg Anti-MBP nanobody. 1mL affinity gel can precipitate at least 1.2mg MBP fusion protein. |
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Components |
1mL Anti-MBP affinity gel, stored in 1mL PBS containing preservative and 50% glycerol. |
Storage
-20°C, 12 months.