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Recombinant Human IL-6/Interleukin-6 Protein (Active)
SKU: PKSH033611-100
Recombinant Human IL-6/Interleukin-6 Protein (Active)
| SKU # | PKSH033611 |
| Expression Host | E.coli |
Description
| Synonyms | IFNB, Interleukin HP, Interferon Beta, B-Cell Stimulatory Factor, IFN-Beta, B-cell hybridoma growth factor, B-Cell Stimulatory Factor 2, BSF2, BSF-2, CDF, CTL Differentiation Factor, HGF, HSF, Hybridoma Growth Factor, IFN-Beta-2, IL-6, Interferon Beta-2, Interleukin 6, Interleukin HP-1, Interleukin-6, IL6, IFNB2, B cell differentiation factor, B cell stimulatory factor 2, BSF 2, Hepatocyte stimulatory factor, Hybridoma growth factor Interferon beta-2, IL 6, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Val30-Met212 |
| Accession | P05231 |
| Calculated Molecular Weight | 21.8 kDa |
| Observed Molecular Weight | 22 kDa |
| Tag | C-His |
| Bio-activity | 1. Measure by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is <0.5 ng/mL. The specific activity of recombinant human IL-6 is approximately >5 x 108 IU/mg. 2. Measure by its ability to induce proliferation in MCF-7 cells. The ED50 for this effect is <4.4 ng/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.