Total Price: $0.00
Contact Information
Acetyl-CoA Carboxylase (ACC) Activity Assay Kit
SKU: E-BC-K508-M-96
Acetyl-CoA Carboxylase (ACC) Activity Assay Kit
| SKU # | E-BC-K508-M |
| Detection Instrument | Microplate reader (650-670 nm, optimum wavelength: 660 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | ACC |
| Sample type | Animal, plant tissue |
| Sensitivity | 6.78 U/L |
| Detection range | 6.78-138 U/L |
| Detection Method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 70 min |
| Precision | Average inter-assay CV: 8% | Average intra-assay CV: 4% |
| Other instruments required | Vortex mixer, Incubator, Centrifuge, 100°C water bath |
| Other reagents required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | -20℃ |
| Valid period | 12 months |
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Potato tissue homogenate | 1 |
Note: The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
ACC catalyzes acetyl-coA, NaHCO3 and ATP to produce inorganic phosphorus, the activity of ACC can be calculated by measuring the change of inorganic phosphorus content.
Kit Components & Storage
| Item | Component | Size (96 T) | Storage |
| Reagent 1 | Buffer Solution | 30 mL × 1 vial | -20°C, 12 months |
| Reagent 2 | Substrate A | Powder × 2 vials | -20°C, 12 months shading light |
| Reagent 3 | Negative Control Solution | 3.5 mL × 1 vial | -20°C, 12 months |
| Reagent 4 | Substrate B | 3.5 mL × 1 vial | -20°C, 12 months shading light |
| Reagent 5 | Chromogenic Agent A | Powder × 2 vials | -20°C, 12 months shading light |
| Reagent 6 | Chromogenic Agent B | Powder × 2 vials | -20°C, 12 months shading light |
| Reagent 7 | Acid Solution | 1.8 mL × 1 vial | -20°C, 12 months |
| Reagent 8 | 500 μmol/L Standard Solution |
6 mL × 1 vial | -20°C, 12 months |
| Microplate | 96 wells | No requirement | |
| Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 12.80 | 56.50 | 103.00 |
| %CV | 4.2 | 4.0 | 3.8 |
Inter-assay Precision
Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 12.80 | 56.50 | 103.00 |
| %CV | 7.4 | 8.2 | 8.4 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 98%.
| Standard 1 | Standard 2 | Standard 3 | |
| Expected Conc. (μmol/L) | 126 | 265 | 438 |
| Observed Conc. (μmol/L) | 127.3 | 251.8 | 429.2 |
| Recovery rate (%) | 101 | 95 | 98 |
Sensitivity
The analytical sensitivity of the assay is 6.78 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
| Concentration (μmol/L) | 0 | 100 | 150 | 200 | 300 | 400 | 450 | 500 |
| OD value | 0.040 | 0.181 | 0.243 | 0.315 | 0.452 | 0.596 | 0.670 | 0.733 |
| 0.040 | 0.172 | 0.242 | 0.312 | 0.458 | 0.599 | 0.671 | 0.744 | |
| Average OD | 0.040 | 0.177 | 0.243 | 0.314 | 0.455 | 0.598 | 0.671 | 0.739 |
| Absoluted OD | 0.000 | 0.137 | 0.203 | 0.274 | 0.415 | 0.558 | 0.631 | 0.699 |