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Angiotensin Converting Enzyme (ACE) Activity Assay Kit
SKU: E-BC-K003-S-100
Angiotensin Converting Enzyme (ACE) Activity Assay Kit
| SKU # | E-BC-K003-S |
| Detection Instrument | Spectrophotometer(340nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | ACE1 |
| Sample Type | Serum, plasma, animal tissue |
| Sensitivity | 27.5 U/L |
| Detection Range | 27.5- 682 U/L |
| Detection Method | Colorimetric method |
| Assay Time | 10-90 min |
| Precision | Inter-assay CV: 4.4% | intra-assay CV: 3% |
| Other Reagents Required | PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| Valid period | 12 months |
Dilution Of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 3-5 |
The diluent is normal saline. For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG) have the maximum absorption peak at 340 nm, angiotensin converting enzyme catalyze N-(3[2-Furyl ]Acryloyl)-Phe-Gly-Gly to produce FAP and GG, and the absorbance at 340 nm will be decreased. The activity of ACE1 can be calculated indirectly by measuring the decrease in absorbance at 340 nm.
Kit Components & Storage
| Item | Component | Size 2 (100 Assays) | Storage |
| Reagent 1 | Working Solution | 55 mL ×2 vials | 2-8°C, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
|
Mean (U/L) |
35.00 | 120.00 | 350.00 |
| %CV | 3.2 | 2.8 | 3.0 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
|
Mean (U/L) |
35.00 | 120.00 | 350.00 |
| %CV | 4.3 | 4.6 | 4.3 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 103%.
| Standard 1 | Standard 2 | Standard 3 | |
| Expected Conc. (U/L) | 80 | 230 | 600 |
| Observed Conc. (U/L) | 80.8 | 241.5 | 618.0 |
| Recovery rate (%) | 101 | 105 | 103 |
Sensitivity
The analytical sensitivity of the assay is 27.5 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.